Dr. Dennis Hooper is an accomplished Carrollton, Texas, medical researcher who directs studies of molds and mycotoxins with Medical Services Consultation, International, LLC. Among the papers Dr. Dennis Hooper has coauthored is “Assessment of Aspergillus fumigatus in Guinea Pig Bronchoalveolar Lavages and Pulmonary Tissue by Culture and Realtime Polymerase Chain Reaction Studies,” which was published in the International Journal of Molecular Sciences in 2012.
The research informing the published findings involved quantitative, real-time analysis of DNA extracted from guinea pigs experimentally infected with the fungus Aspergillus fumigatus (AF) that used the presence of AF DNA as a diagnostic tool. DNA and respiratory tissue and fluids were extracted from the guinea pigs and cultured. Quantitative measures were used in looking at specimens of the respiratory tissue, with the quantity of spores in AF-infected tissues being compared with those in the aerosolized samples that were employed in inoculating the guinea pigs.
The guinea pig DNA was also examined for the presence of AF DNA. The key findings were that the absence or presence of AF DNA could be determined through the specific probe and primer set. Dr. Hooper and his colleagues demonstrated that real-time PCR, in combination with DNA primers and hydrolysis probes, was effective in diagnosing early-stage fungal infections.
The research informing the published findings involved quantitative, real-time analysis of DNA extracted from guinea pigs experimentally infected with the fungus Aspergillus fumigatus (AF) that used the presence of AF DNA as a diagnostic tool. DNA and respiratory tissue and fluids were extracted from the guinea pigs and cultured. Quantitative measures were used in looking at specimens of the respiratory tissue, with the quantity of spores in AF-infected tissues being compared with those in the aerosolized samples that were employed in inoculating the guinea pigs.
The guinea pig DNA was also examined for the presence of AF DNA. The key findings were that the absence or presence of AF DNA could be determined through the specific probe and primer set. Dr. Hooper and his colleagues demonstrated that real-time PCR, in combination with DNA primers and hydrolysis probes, was effective in diagnosing early-stage fungal infections.